Sun Protective activity of the hydroalchoholic
extracts of two Medicinal Flowers
S.B. Patil1*, M.
Y. Lende1, V.
S. Thakur1,
N.S. Naikwade1 and
C.S. Magdum2 and R. D. Bendgude3
1Department
of Pharmacology, Appasaheb Birnale
College of Pharmacy, South Shivaji Nagar, Sangli Maharashtra, India – 416 416
2Rajarambapu
College of Pharmacy, Kasegaon, Sangli,
Maharashtra, India
3SVERI’S College of Pharmacy, Pandharpur, Maharashtra, India
*Corresponding author E-mail: sandeep_pharmacology@rediffmail.com
ABSTRACT:
Sunscreens are
chemicals that provide protection against the adverse effects of solar and in
particular UV radiation. In this present study UV
absorption ability of flowers evaluated from flowers of Thunburgia alata
Linn, Abutilon indicum Linn. Extracts were prepared by maceration with a mixture of
distilled water and methanol (1:1). The method is performed by UV spectrophotomety in the range of 200 to 400 nm and results
of all the extracts shows effective UV absorption in the given range.
KEYWORDS: UV protective,
Thunburgia alata
Linn, Abutilon indicum Linn, hydroalcoholic.
INTRODUCTION:
Sun radiation
constantly impacts the earth with approximately 50% visible light (400- 800
nm), 40% infrared radiation (IR) (1300- 1700 nm), and 10% ultraviolet radiation
(UV) (10-400 nm). UV is divided conventionally to UV-A (320-400 nm), UV-B
(290-320 nm), UVC (100-290 nm), and vacuo UV (10-100
nm). Sunscreens are chemicals that provide protection against the adverse
effects of solar and in particular UV radiation. But due to high cost and
allergy of marketed sunscreen lotions so the alternative use of herbal drugs
are now day’s necessarily1.
Abutilon indicum
L., (Malvaceae) commonly known as “Atibala” in Sanskrit gives excessive tonic strength.
Flowers and leaves were used as local application to boils and ulcers and also
as an emollient. Also a stronger diuretic and heart tonic2. Chemically it contains flavonoids
(quercetin), saponins,
alkaloids and phenolic compounds3.
Thunbergia
alata L or black-eyed susan, is well known as a
fast-growing, long-flowering, friendly creeper. Medicinally it is used for skin
problems, cellulitis, back and joint pains, eye
inflammation, piles and rectal cancer. Some people can get contact dermatitis
from it4.
EXPERIMENTAL:
Plants:
Thunburgia
alata L, Abutilon indicum L., flowers were freshly obtained from local region at Sangli. Botanical identification was performed at the
Department of Botany by approved botanist at Padmbhushan
Dr. Vasantdada Patil Mahavidyalay, Tasgaon, Dist Sangli, Maharashtra, India.
Preparation
of Extract:
Later
the petals were separated and dried. 100 g powdered petals were extracted with
Distilled water: methanol (1:1) by maceration. The extracts were evaporated to
dryness. Yield was obtained 1.8
gm, 1.7 gm respectively.
The
general flavonoids identification test was performed
on all the four extracts5.
Test
1 (Shinoda test): To dry extract, add 5 ml 95%
ethanol, few drops concentrated hydrochloric acid and 0.5 g magnesium turnings.
Pink colour observed.
Test
2: To small quantity of extract, add lead acetate solution, yellow coloured precipitate is formed.
Preparation
of sample:
Samples
were prepared in 8 mg % w/v concentration by using distilled water (8 mg / 100
ml).UV absorption spectra for all the two extracts were obtained in the range
of 200-400 nm using Jasco UV spectrophotometer.
Following
graphs indicates absorption spectra for all the 2 extracts in the given range.
Scanning
spectra of extracts on UV-spectrophotometer.
Figure 1: indicates the spectrum obtained from Hydro-alcholic extract of Thunburgia
alata Linn.
Figure 2: indicated the spectrum obtained from Hydro-alcholic extract of Abutilon indicum
Linn.
RESULT AND DISCUSSION:
In this study,
two plants studied, both plants had sun protective activity by absorbing UV C rays which damage to the skin and hence
both extract of flower proved its UV protection ability. Both extract showed a
prominent absorbance at 200 – 230 nm. Phytochemical investigation indicated the
presence of flavonoids in both extracts and this
protection ability may observe due to the presence of flavonoids.
REFERENCES:
1. Payam Khazaeli and Mitra Mehrabani, Screening of Sun
Protective Activity of the Ethyl Acetate Extracts of Some Medicinal Plants, Iranian
Journal of Pharmaceutical Research 7 (1);2008: 5-9
2.
Kirtikar KR., Basu BD. Indian Medicinal Plants. 2nded. Vol.2,
Allahabad, I: Lalit Mohan Basu,
1961. P.314-315.
3.
Gaind KN., Chopra KS.,
Phytochemical investigation of Abutilon indicum,
Planta Med., 30(2); 1976: 174-88.
4.
(http://www.plantzafrica.com/planttuv/thunbergalata.htm)
5.
Khandelwal
KR Practical Pharmacognosy, 12th
Edition, Nirali Prakashan, Pune. 2004 pp. 153.
Received on 23.12.2012 Accepted on 08.01.2012
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Asian J. Pharm.
Res. 2(1): Jan.-Mar. 2012;
Page 37-38